Up to March 26, 2010



The last day to receive your Abstract is April 30 th, 2010
Preferred presentation    
  You can choose the kind of presentation you want, and include this preference in your Abstract  
  Keynote presentation (decided by the Organizing Committee)    
  Oral presentation    
  We will have a personal computer (PC) with Power Point 2003 for the projection. If you need other resources please contact us as soon as possible at mptoxins2009@ibiologia.unam.mx  
  You will have to be in front of your poster, in the specified timetable, to answer all the questions. The information about posters is at the last part of this section.  
The final decision will be given by the Scientific Committee    
  The official language of the congress is English, but Spanish and Portuguese can be used in posters, although their explanations in English facilitate the communication among dozens of countries.  

Abstracts will have a maximum two pages. Keynote presentations a maximum of 10 pages. Abstract must be sent in Word Office 2003 or 2007, justified, with the following format sent to the e-mail:


  Title: Arial 14 points, bold, capital letters.  

Authors: First name, last name of authors and the corresponding author with *. Telephone and e-mail of the corresponding author. Arial 12 points, bold.

Addresses: Department, Institution, City, Postal code, Country. Arial 12 points.

Background: Arial, 12 points.

Aims: Arial, 12 points.

Materials and Methods: Arial, 12 points.

Results and Discussion: Arial, 12 points.

Conclusions: Arial, 12 points.

References: Arial, 12 points.



Mariana Díaz-Zaragoza, Magda Carvajal*, Ignacio Méndez, Nahlleli Civi-Chilpa,
Ernesto Ávila- González, César Flores. 

Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, 04510 México, D,F.
 *Tel: 5255+ 5622-9138    magdac@servidor.unam.mx

Background:Aflatoxins (AF) are secondary metabolites produced by strains of the fungi Aspergillus flavus, A. parasiticus and A. nomius, and they turn out to be a natural and dangerous contaminant of several cereal grains such as maize and sorghum. They are a great risk for both human and animal health. There are four important AF: AFB1, AFB2, AFG1 and AFG2, from which AFB1 is the most toxic, and it is considered a potent mutagen and carcinogen. AF are the most important mycotoxins in the broiler industry because they cause great economic losses, when poultry are fed with contaminated grains.

Aim: To quantify and identify AF in liver of laying hen, and to determine the best chemical method for their AF detection.

Materials and Methods: A total of 25 laying hens of 121-week old in a second cycle of egg production, were distributed at random in three experimental groups and placed in individual cages. The control group had 9 hens fed with 2 kg of milled and homogenized clean sorghum, without AFB1, every day for one week; and of two treated groups of 8 hens each,  fed with contaminated sorghum with a low (30 µg/kg) or a high (500 µg/kg) AF doses. After a week, the hens were killed by cervical dislocation and their livers were removed. The extraction method for AF was the one proposed by Qian & Yang (1984), and the chemical method with immunoaffinity columns given by Koeltzow & Tanner (1990). The quantification of AF was achieved by high-performance liquid chromatography (HPLC). The liver was the tissue with higher contamination and number of identified AF.

Results and Discussion: The livers had AFB1 (0.7-9.7 µg/kg), AFB2 (0.06-0.6 µg/kg), AFG1 (78.7-121.0 µg/kg), AFG2 (16.2-18.7 µg/kg), AFM1 (10.3-591.8 µg/kg), AFP1 (96.2-771.3 µg/kg) and AFL (4.8-12.6 µg/kg), from which AFG1, AFG2, AFM1 and AFP1 were obtained in greater amount. The determination of the appropriate chemical method for the quantification of AF settled down when the percentage of recovery of both methods was made. In both chemical methods around 80% of recovery was obtained which is an acceptable recovery. Nevertheless, the method of Qian & Yang (1984) had a lower limit of detection (0.5 ng/mL), while the limit of detection in the chemical method with immunoaffinity columns was of 5 ng/mL for liver.

Conclusion: In conclusion, the best chemical method for the quantification of AF, was the proposed by Qian & Yang (1984), however, it requires a modification to obtain a recovery of 80 to 90%, which consists of the addition of both AF elutions, from the first and second Supelclean LC-18 SPE columns. 

Qian, G-S & Yang, G. C. 1984. Rapid extraction and detection of aflatoxins B1 and M1 in beef liver. Journal Agricultural and Food Chemistry, 32:1071-1073.

Koeltzow, D. E. & Tanner, S. N. 1990. Comparative evaluation of commercially available test methods. Journal Association of Official Analytical Chemists, 73: 584-589.

  90 width x 120 cms height. Free style.  
  Authors: First Name, Last name of authors and the corresponding author with * Telephone and e-mail of the corresponding author.  
  Addresses:Department, Institution, City, Postal code, Country.